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. 2022 Apr 7;13(4):314. doi: 10.1038/s41419-022-04736-6

Fig. 4. Relationship between miR-344d-3-5p and Prc1 (and Nuf2).

Fig. 4

A, B Relative luciferase activities of wild type (WT) and mutated (MUT) Prc1 reporter gene vectors in B16 cells co-transfected with miR-344d-3-5p mimic/miR-344d-3-5p mut. C, D Relative luciferase activities of wild-type (WT) and mutated (MUT) Nuf2 reporter gene vectors in B16 cells co-transfected with miR-344d-3-5p mimics/miR−344d-3-5p mut. E PRC1 protein expression following transfection with miR-344d-3-5p mimics/miR-344d-3-5p mut. F Quantitative analysis of the protein level of PRC1 in miR-344d-3-5p mimics/miR-344d-3-5p mut-transfected B16 cells. G NUF2 protein expression when transfected with miR-344d-3-5p mimics/miR-344d-3-5p mut. H Quantitative analysis of the protein level of NUF2 in miR-344d-3-5p mimics/miR-344d-3-5p mut-transfected B16 cells. I Quantitative analysis of the mRNA level of Prc1 in B16 cells after treated with ATRA and PB-4 by RT-qPCR. J PRC1 protein expression in B16 cells after treatment with ATRA and PB-4. K Quantitative analysis of the protein level of PRC1 in B16 cells following treatment with ATRA and PB-4. L Quantitative analysis of the mRNA level of Nuf2 in B16 cells after treatment with ATRA and PB-4 by RT-qPCR. M NUF2 protein expression in B16 cells after treatment with ATRA and PB-4. N Quantitative analysis of the protein level of NUF2 in B16 cells after treatment with ATRA and PB-4. n = 3. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01 compared with the corresponding control group by Student’s t-test.