Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 7;12:5850. doi: 10.1038/s41598-022-09882-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effects of GBCAs on focal adhesion and F-actin formation in astrocytes. (A) Representative photomicrographs showing the effects of GBCAs on cell adhesion assays using astrocytes. Cells were stained with cresyl violet (Sigma). Quantitative analysis of the effect of Omniscan (B), Magnescope (C), Magnevist (D), and Gadovist (E) (1–100 nM) on cell adhesion. (F) Quantitative analysis of the effect of GBCAs after coexposure with integrin inhibitor on cell adhesion assays. Mouse primary cerebral cortex astrocytes were cultured for seven days, followed by fluorescence labeling analysis using phalloidin F-actin and DAPI. Astrocytes were exposed to GBCAs and/or cRGDfV for 60 min after serum-starvation for 6 h. (G) Representative photomicrographs showing the F-actin and DAPI staining to examine the effects of GBCA on cortical actin stress fibers formation. (H) Changes in the cortical F-actin score index after coexposure with cRGDfV. Bars represent 50 μm. (I) Representative blots of talin-1, vinculin, α-actinin, cortactin, paxillin, and GAPDH levels of 60 min GBCAs exposure after knockdown of ITGAV and ITGB3 in U87MG cells. Quantitative analysis of GBCAs effect on the protein expression levels of talin-1 (J), vinculin (K), α-actinin (L), cortactin (M), and paxillin (N). Protein band intensities (single band for talin-1, vinculin, α-actinin, and GAPDH; two bands for cortactin; three bands for paxillin) were quantified using Fiji ImageJ software (NIH). Bars represent 50 μm. Data are expressed as the mean ± SEM of at least three independent experiments. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, indicates statistical significance were analysed by two-way ANOVA continued with post hoc Bonferroni’s or Turkey test compared with the control. ^####p < 0.0001, ^###p < 0.001, ^##p < 0.01, ^#p < 0.05, indicates statistical were analysed by two-way ANOVA continued with post hoc Bonferroni’s or Turkey.