FIG. 5.

Stability of Hsn-5 (H-5) and MUC7 D1 (M-D1) in human serum at 37°C, without or with protease inhibitors, as monitored by 15% cationic PAGE (A) and SDS-PAGE (B), after incubation for 2 h. Lane 1, serum (Ser) (unincubated); lanes 2 and 7, serum (37°C); lanes 3 and 8, Hsn-5 (37°C); lanes 4 and 9, Hsn-5 plus serum (37°C); lanes 5 and 10, MUC7 D1 (37°C); lanes 6 and 11, MUC7 D1 plus serum (37°C). Protease inhibitors were added to the serum used in lanes 7 to 11. Arrowheads are as in Fig. 4. Lane M is as in Fig. 4.