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Transfection efficiency of liposomes. HEK-293T cells were transfected by pDNA (Lenti-Luciferase-IRES-ZsGreen) with indicated lipoplexes; the expression of ZsGreen proteins was imaged by using a fluorescent microscope (A), and we quantified percentage of ZsGreen positive cells (B) and MFI (C) by flow cytometry at 48 h post transfection. At 48 h post transfection, cells were trypsinized and stained with 7-AAD dye and estimated the cell viability by flow cytometry (D).
