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. 2022 Mar 25;14:848991. doi: 10.3389/fnagi.2022.848991

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Copyright © 2022 Cazzaniga, Bistaffa, De Luca, Portaleone, Catania, Redaelli, Tramacere, Bufano, Rossi, Caroppo, Giovagnoli, Tiraboschi, Di Fede, Eleopra, Devigili, Elia, Cilia, Fiorini, Bongianni, Salzano, Celauro, Quarta, Mammana, Legname, Tagliavini, Parchi, Zanusso, Giaccone and Moda.

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Analysis of brain homogenates by PMCA. Wb of the 3^rd and 6^th PMCA rounds are shown. After 6 rounds of amplification, PrP^res was detected in the brains of (A) 5/7 MM1, (B) 2/3 MM2C, (C) 2/2 MM2T, (D) 3/3MV1, (E) 5/6 MV2, (F) 0/1 VV1, and (G) 4/4 VV2, although with variable efficiency. (H) No PrP^res was amplified from the brain of OND patients (AD and FTD). (A–C) In particular, type 2 PrP^res with a prevalence of the di-glycosylated species was generated by the BHs of 4/7 MM1, 2/3 MM2C and 2/2 MM2T patients, except for one MM1 patient (7_BH) in which the presence of a type 1 PrP^res with an equal representation of the di- and mono-glycosylated band was observed. (D–G) In contrast, type 1 PrP^res was generated by BHs of 3/3 MV1, 5/6 MV2 patients and 4/4 VV2 patients. Except for 2 VV2 samples (25_BH and 26_BH), all amplified PrP^res were characterized by a prevalent di-glycosylated band. Numbers in the right of each Wb indicate the molecular weight marker.