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. 2022 Mar 25;14:848991. doi: 10.3389/fnagi.2022.848991

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Copyright © 2022 Cazzaniga, Bistaffa, De Luca, Portaleone, Catania, Redaelli, Tramacere, Bufano, Rossi, Caroppo, Giovagnoli, Tiraboschi, Di Fede, Eleopra, Devigili, Elia, Cilia, Fiorini, Bongianni, Salzano, Celauro, Quarta, Mammana, Legname, Tagliavini, Parchi, Zanusso, Giaccone and Moda.

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Quantitative PMCA (qPMCA) for estimating PrP^res concentration in OM samples of sCJD patients. (A) Serial dilutions of recombinant full-length human PrP (recHuPrP_23–231) were used to estimate prion concentration in the brain of sCJD patients. (B) Serial dilutions of sCJD brain homogenates subjected to PK and PNGase treatments before Wb analysis. Quantitative PMCA to estimate PrP^res concentration in OM of (C) MM1, (D) MV2, and (E) VV2 patients. Specific rounds at which every OM PrP^res was detected (3^rd for the MM1 and one VV2, 5^th for one MV2 and one VV2, and 6^th for one MV2) are shown.