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. 2022 Mar 25;10:856398. doi: 10.3389/fbioe.2022.856398

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Copyright © 2022 Ze, Li, Huang, Shi, Li, Gong, Lin and Yao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Printing of highly vascularized tissues with high cell density. (A) Schematic diagram of the indirect 3D bioprinting workflow. (B) Organ building block (OBB) tissue matrix formation. (C,D) Sacrificial ink writing within an embryoid body (EB) matrix. (E) Examples of different OBB-based matrices. (F) Fabrication of a helical vascular structure in an EB matrix. Reproduced with permission (Skylar-Scott et al., 2019). Copyright 2019, American Association for the Advancement of Science (AAAS).