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. 2022 Apr 8;12:5936. doi: 10.1038/s41598-022-08263-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Process flow from saliva collection to data reporting. (A) Schematic diagram of the main steps of the process flow. (B) Sentinel robot for LAMP reaction setup, incubation, and detection. Individual process steps: 1. Saliva collection via microswabs into test tubes containing inactivation solution. 2. Sample tubes with saliva dilutions were heat-inactivated for 10 min at 95 °C. 3. Automated uncapping of a complete sample tube rack. 4. Sample tube barcode reading during Sentinel robot loading. 5. The automated pipetting system adds a selected sample volume to the RT-LAMP master mix in clear reagent plates. 6. Plate sealing by automated heat sealer. 7. Plate crane transport of the reaction plate onto a 65 °C incubation field. 8. LAMP reactions were incubated at 65 °C for 25 min; digital, parallel scanning of multiple 96-well microplates. 9. Real-time, secure reporting of deidentified data and analysis. 10. Disposal of scanned microplates.