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. 2022 Mar 1;113(4):1393–1405. doi: 10.1111/cas.15302

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© 2022 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Expression of TRAF6 and lymphatic vessels in colorectal cancer clinical samples. (A, B) Representative immunohistochemical staining images for TRAF6 protein and D2‐40 in colorectal cancer (CRC) and adjacent normal tissues from a tissue array are shown. Magnification, ×40 and ×100. (C) Mean optical density (MOD) showed that TRAF6 expression in colorectal cancer tissues was elevated compared with normal colorectal tissues. (D) The result of LVD showed that the expression of lymphatic vessels in colorectal cancer tissues was elevated compared with normal colorectal tissues. (E) Representative immunohistochemical staining images for TRAF6 protein in CRC tissues from a tissue array were shown. Magnification, ×100 and ×200. (F) The result of immunohistochemical staining showed that the expression of relative TRAF6 expression levels in N1 and N2 stage colorectal cancer tissues was elevated compared with N0. Data are shown as mean ±SD. ***P < 0.001