TABLE 2.
Nucleotide sequence of the blaTEM-68 coding region compared with those of the blaTEM-1a, blaTEM-1b, blaTEM-2, blaTEM-47, blaTEM-48, and blaTEM-49 genes
| Nucleotidea (amino acidbc) position | Nucleotide (amino acid)d
|
||||||
|---|---|---|---|---|---|---|---|
| blaTEM-1ae | blaTEM-1bf | blaTEM-2f | blaTEM-48g | blaTEM-49g | blaTEM-47g | blaTEM-68 | |
| 226 | C | T | T | T | |||
| 263 (21) | C (Leu) | T (Phe) | T (Phe) | ||||
| 317 (39) | C (Gln) | A (Lys) | |||||
| 346 | A | G | G | G | |||
| 436 | C | T | T | T | T | T | T |
| 512 (104) | G (Glu) | ||||||
| 604 | G | T | |||||
| 682 | T | C | C | C | C | C | |
| 692 (164) | C (Arg) | ||||||
| 914 (238) | G (Gly) | A (Ser) | A (Ser) | A (Ser) | A (Ser) | ||
| 917 (240) | G (Glu) | A (Lys) | A (Lys) | A (Lys) | A (Lys) | ||
| 925 | G | A | A | A | A | A | |
| 990 (265) | C (Thr) | T (Met) | T (Met) | T (Met) | T (Met) | ||
| 998 (268) | A (Ser) | G (Gly) | |||||
| 1020 (275) | G (Arg) | T (Leu) | |||||
a
Nucleotide positions are numbered as described by Sutcliffe (42).
b
Amino acid positions are numbered as described by Ambler et al. (1).
c
Amino acid numbers are indicated only for positions at which substitutions are observed.
d
Only mutational changes with respect to the TEM-1 β-lactamase and/or blaTEM-1a gene sequences are listed.
e
Reference 42.
g
Reference 15.