FIGURE 4.

Effect of the benzothiazepine S107. In ScreenChip™ recordings of EPGs under blue light stimulation (4 Hz, 50 ms), the effect of S107 (50 μM, 30 min pre-incubation, shaded boxes) in comparison to the vehicle DMSO (0.1% in M9 buffer) on pump rate of UNC-68 mutants S2378L (A), P2460S (B) and Q4623R (C) was determined. Alleles have been crossed with a fkb-2 deletion [fkb-2(ok3007), violet boxes] to check for drug-induced UNC-68 stabilization via FKB-2. S107 rescues UNC-68 function of Q4623R and P2460S only in FKB-2 wt background. Box plots include mean (open circle) and median (line). Significance levels (asterisks), referring to respective controls (wild type, either with or without S107), are located above the corresponding boxes. Comparison of each strain with and without S107 treatment is indicated above the line pointing on the respective boxes. N-numbers as indicated. One-way ANOVA with the Bonferroni post-hoc test was performed: n.s., not significant, *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001.