FIGURE 7.

Characterization of neutral amino acid insertion into UNC-68 luminal loop (lum. loop). (A,B) No effect on spontaneous pumping on food and reduced number of swimming cycles/min evoked by neutral luminal loop insertion. (C) Pumping ability is not reduced in the ScreenChip™ system (4 Hz stimulation, 60 s). (D) No effect on maximal pump rate of cut-head preparations in the stress test. (E) Exemplary false-color representation of fluorescence intensity. (F) Mean ± SEM of the ΔF/F₀ of RCaMP1h fluorescence before and during light stimulation. (G) The amplitudes of ΔF/F₀ (mean: 10–13 s) show no significant difference between wild-type and neutral luminal loop-expressing animals. Bar graphs represent mean ± SEM, box plots include data points (rhomb), mean (circle), and median (line). N-numbers and significance levels (asterisks) are displayed. One-way ANOVA with the Bonferroni test (A,B) or a two-sample t-test (C,D,G): n.s., not significant, and ***P ≤ 0.001.