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GFP expression in parthenogenetic embryos of B. mori 1–31 incubated at 15°C illustrating efficiency of microinjection at different developmental stages. The GFP-containing DNA plasmid was injected into the heat-shock-activated eggs during the first 72 h AEA in times specify by numbers in each figure. GFP expression was examined 4 days before hatching. Time AEA is indicated by the inset numbers. Scale bar: 500 μm.
