Skip to main content
. 2022 Mar 25;13:822900. doi: 10.3389/fphys.2022.822900

TABLE 2.

Stages of early embryogenesis in B. mori embryos incubated at 15°C.

Timing after egg activation (at 15°C) PK1
Implant PK1 in K23 ♂
K23
Stage % n Stage % n Stage % n
12 h A 100 12 A 100 16 A 100 11
24 h B 100 16 B 100 14 B-C 40–60 12
36 h C-D 40–60 10 C-D 75–25 15 D-E 80–20 10
48 h D-E 10–90 11 D-E 65–35 16 E-F 80–20 27
60 h F 100 14 F 100 2 F 100 12
72 h F 100 10 F 100 9 F 100 21

Embryonic development was analyzed at 12-h intervals during the first 72 h AEA. Each time point is characterized by developmental stages A-G described in Table 1. PK1, parthenogenetic eggs from PK1 ovaries; implant PK1 in K23 ♂, parthenogenetic PK1 eggs from ovarian implants that developed in K23 males; K23, fertilized eggs from endogenous K23 ovaries; n, number of embryos examined; %, percentage of occurrence of the embryonic developmental stage examined among all eggs in the sample. Stages of embryonic development were determined subjectively by two observers.