TABLE 2.
Stages of early embryogenesis in B. mori embryos incubated at 15°C.
| Timing after egg activation (at 15°C) | PK1 |
Implant PK1 in K23 ♂ |
K23 |
||||||
| Stage | % | n | Stage | % | n | Stage | % | n | |
| 12 h | A | 100 | 12 | A | 100 | 16 | A | 100 | 11 |
| 24 h | B | 100 | 16 | B | 100 | 14 | B-C | 40–60 | 12 |
| 36 h | C-D | 40–60 | 10 | C-D | 75–25 | 15 | D-E | 80–20 | 10 |
| 48 h | D-E | 10–90 | 11 | D-E | 65–35 | 16 | E-F | 80–20 | 27 |
| 60 h | F | 100 | 14 | F | 100 | 2 | F | 100 | 12 |
| 72 h | F | 100 | 10 | F | 100 | 9 | F | 100 | 21 |
Embryonic development was analyzed at 12-h intervals during the first 72 h AEA. Each time point is characterized by developmental stages A-G described in Table 1. PK1, parthenogenetic eggs from PK1 ovaries; implant PK1 in K23 ♂, parthenogenetic PK1 eggs from ovarian implants that developed in K23 males; K23, fertilized eggs from endogenous K23 ovaries; n, number of embryos examined; %, percentage of occurrence of the embryonic developmental stage examined among all eggs in the sample. Stages of embryonic development were determined subjectively by two observers.