Figure 1.

(A) Schematic illustration of the sequence of steps involved in enzyme immobilization: surface activation by plasma treatment; amine (NH2) functionalization with 3-aminopropyltrimethoxysilane (APTMS) via silanization; activation of the catheter lumen surface using the bifunctional agent (GDA) under the formation of Schiff base and finally coupling of the enzyme. (B) Chemical immobilization of PslG_h prevents P. aeruginosa biofilm formation on the luminal surface of catheter tubing. Schematic representation demonstrating cell attachment and biofilm formation on untreated catheters (top) and inhibition of biofilm formation when the catheter is treated with PslG_h (bottom).