Antibiofilm activity of the lumen of high density PslGh-bound 10 cm PE-100 catheters against P. aeruginosa. (A) Photos of CV stained 10 cm PE-100 catheters; (B) Fluorescence images of 5 alternating segments (i.e., S2, 4, 6, 8 and 10); and (C) Colony forming units per cm2 (CFU) of the other 5 alternating segments (i.e., S1, 3, 5, 7 and 9). All measurements were acquired after incubation for 24 h in bacterial culture. Biofilms were stained with CV in (A) and with SYTOX Green in (B) (scale bar, 50 μm). *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001.