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. 2022 Mar 28;13(6):2029–2039. doi: 10.7150/jca.63533

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NRCAM is the target gene of miR-338-3p in osteosarcoma. (A) Nine common target genes were discovered across the DEGs' overlapping analysis from TCGA and GSE12865 with predicted target genes. (B, C) The nine DEGs' heatmap were showed in GSE12865 set as well as TCGA database, respectively. (D) The six target genes that were obviously raised in both sets were assessed by qRT-PCR assay in MNNG/HOS, MG-63 and hFOB1.19 cells. (E) Western blot assay for NRCAM with miR-338-3p mimics. (F) The descriptive diagram for the binding site of miR-338-3p to NRCAM's 3'-UTR. (G) pMIR-REPORT luciferase vector comprising of NRCAM 3'UTR or a mutated type was co-transfected in osteosarcoma cells with miR-338-3p mimics or inhibitors or miR-NC. Data was presented as mean ± SD from three independent experiments. *, P <0.05; ns, not significant.