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. 2022 Mar 28;13(6):1958–1971. doi: 10.7150/jca.69497

Figure 2.

Figure 2

Construction of the CCT3 knockdown melanoma cell model. (A) Infection efficiency determined by light and fluorescence microscopy at 72 h following lentiviral infection in A375 and MUM-2C cells. Representative images of the cultures are shown (original magnification, 200×). (B) CCT3 mRNA levels in A375 and MUM-2C cells after shCCT3 lentivirus infection measured by qRT-PCR. Data are presented as mean ± SD (n = 3). **P < 0.01, shCtrl vs. shCCT3. (C) CCT3 protein expression in the shCCT3 lentivirus-infected A375 and MUM-2C cells analyzed by Western blotting. GAPDH was used as internal control.