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. 2022 Mar 28;13(6):1958–1971. doi: 10.7150/jca.69497

Figure 4.

Figure 4

CCT3 knockdown induces the cell apoptosis of melanoma cells and influences the distribution of cell cycle. (A) Flow cytometry analysis based on the annexin V-APC staining for the detection of the percentage of early apoptotic cells in A375 and MUM-2C cells. Region R3 spans the fluorescence density of apoptotic cells. Representative images for each group are shown. (B) Quantification of early apoptotic cells in A375 and MUM-2C cells. (C) Activities of caspase 3/7 of A375 and MUM-2C cells with and without CCT3 knockdown detected using the caspase 3/7 activity assay. (D) Flow cytometry analysis of cell cycle distribution in A375 and MUM-2C cell lines. Representative images for each group are shown. (E) The expression levels of some downstream apoptosis markers in A375 cells by western blot. Band density was normalized to β-actin. Data are expressed as mean ± SD (n=3), shCtrl vs. shCCT3, *P < 0.05, ** P < 0.01, *** P < 0.001.