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. 2022 Mar 28;13(6):1958–1971. doi: 10.7150/jca.69497

Figure 7.

Figure 7

The validation results and changes of cell cycle signal molecules after CCT3 silencing in melanoma cells. (A) qRT-PCR showed the upregulation of 12 genes and downregulation of two genes in CCT3-KD A375 cells. (t test, P < 0.01 for all genes). (B) and (C). Western blotting analysis showed decreased levels of CDK1, FOXM1, MCM2, PIM1, and SKP2 genes and no statistical difference for NFKBIA in CCT3-KD A375 cells. (D). RT-qPCR results indicated that the expression of CDK1 mRNA was successfully rescued after shCCT3 melanoma cells were infected with CDK1 overexpression lentivirus vector (OE-CDK1). (E) MTT assay showed that decreased cell proliferation by CCT3 silencing was rescued by the overexpression of CDK1. *P < 0.05, **P < 0.01, ***P < 0.001, compared with the shCtrl cells.