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. 2022 Mar 28;13(6):1923–1932. doi: 10.7150/jca.66914

Figure 3.

Figure 3

Hdac11 down-regulates the expression level of Mmp3 partly through reducing the level of histone H3K9 acetylation at the promoter of Mmp3. (A) PIRESneo3-HDAC11 was transfected into CT26.WT cells and an empty vector was served as a negative control. The expression of Hdac11 and Mmp3 was tested by qRT-PCR and Western blotting. (B) CT26.WT cells were transfected with siRNA targeting Hdac11 or control siRNA and the expression of Mmp3 was determined by qRT-PCR and Western blotting. (C) The murine macrophages (RAW264.7) were treated with LPS to induce an acute inflammation response, and the indicated proteins are assayed by Western blotting. (D) Comparing the histone occupancy in the Mmp3 promoter between normal and inflammation tissues. Native ChIP assay was used to assay the levels of histone H3K9 and H3K14 acetylation at the Mmp3 promoter in normal and inflammation colon tissues from the AOM/DSS-induced colitis mice model. Error bars represent SD.*p < 0.05, **p < 0.01, ***p < 0.001 compared with control.