Figure 3.

Effect of ONP-302 on gene expression in tumor-associated macrophages (TAMs) and fibroblasts. LLC tumor bearing C57BL/6 mice were treated with vehicle or ONP-302 50mg/kg intravenously twice per week for 2 weeks. Single-cell suspensions were prepared from tumor. TAMs and fibroblasts were sorted by fluorescence activated cell-sorting. A. Effect of ONP-302 treatment on the percentage of TAM (CD11b+F4/80+) in the tumor. B. Effect of ONP-302 on the expression of indicated genes in TAMs assayed by q-PCR. Relative gene expression was normalized to GAPDH. C. Effect of ONP- 302 treatment on the percentage of CAFs (CD45-CD140a+CD326-) in the tumor. Effect of ONP-302 on the expression of indicated genes in CAFs assayed by q-PCR. Relative gene expression was normalized to GAPDH and calculated by the comparative C(T) method ⁴².