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. 2022 Mar 28;13(6):1933–1944. doi: 10.7150/jca.69338

Figure 4.

Figure 4

Evaluation of particle-positive cells in the tumor and spleen. LLC tumor bearing C57BL/6 mice were intravenously treated injected with fluorescently labelled (Alexa-Fluor 647) ONP-302 50mg/kg. 2 hours after injection, single-cell suspensions were prepared from tumor and spleen and ONP-302-positive cells were analyzed by flow cytometry. A. Flow cytometry gating scheme for identifying ONP-302-positive TAMs (CD11b+F4/80+), M-MDSC (CD11b+ Ly6Chi Ly6G-), and PMN-MDSC (CD11b+ Ly6C- Ly6G+), and CAFs (CD45-CD140a+CD326-). B. Frequency of Alexa-Fluor 647-positive TAMs (CD11b+F4/80+), M-MDSC (CD11b+ Ly6Chi Ly6G-), and PMN-MDSC (CD11b+ Ly6C- Ly6G+), and CAFs (CD45- CD140a+CD326-) in LLC tumors. C. Frequency of Alexa-Fluor 647-positive macrophages (CD11b+F4/80+), monocytes (M-MDSC) (CD11b+ Ly6Chi Ly6G-), and neutrophils (PMN-MDSC) (CD11b+ Ly6C- Ly6G+) in spleens of LLC tumor-bearing mice.