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. 2022 Mar 21;18(6):2527–2539. doi: 10.7150/ijbs.69266

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DNMT3A could regulate the expression of TIMP2. (A) TIMP2 was hypermethylated in CRC analyzed from UCLCAN database. (B) 5-Aza promoted the expression of TIMP2 mRNA. (C) ChIP was performed to detected the DNMT3A binding region of TIMP2 promotor. (D) MSP was performed to detected the methylation level of TIMP2 promotor. *P<0.05, **P<0.01, ***P<0.001.