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DNMT3A Partially reversed the effect of TDG in colon cancer cells. (A) MSP was performed to detected the methylation level of TIMP2 promotor. (B) The transwell assay was performed for migration and invasion of colon cancer cells (C+C:Ctrl.+Control, T+C: TDG+Control, C+D: Ctrl.+DNMT3A, T+D: TDG+DNMT3A). (C-D) The expression of TIMP2 was detected by qRT-PCR and western blotting. *P<0.05, **P<0.01, ***P<0.001.
