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. 2022 Mar 14;18(6):2419–2438. doi: 10.7150/ijbs.67200

Figure 7.

Figure 7

AKT/4EBP1/eIF4E enhances PD-L1 translational level in colon cancer with mutant p53. (A) The results of gene set enrichment analysis (GSEA) showed that PD-L1 significantly regulated with the p53 signaling pathway and eIF4E pathway. (B, C) Regarding the enrichment plot p53 signaling pathway and eIF4E pathway. (D, E) The HCT116 and SW480 cells were treated with pifithrin (PFT)-α at 0, 25, 50, 100, 200, and 400 µM after 24 hours. Lysates from these cells were immunoblotted by the indicated antibodies. (F, G) The HCT116 (F) and SW480 (G) cells were treated with PD-1/PD-L1 inhibitor for 24 hours at different doses between 0 and 80, respectively. Lysates from these cells were immunoblotted with indicated antibodies and β-actin was used as a loading control in western blotting analysis.