Elevated ALKBH5 level in MM patient-derived CD138+ cells and MM cell lines. (A) The expression level of ALKBH5 in primary CD138+ cells from MM patients (n=15) and normal volunteers (NCs, n=15) were detected by western blot. Each band represents 5 patients as samples were randomly divided into 3 groups to get enough protein. (B) The expression level of ALKBH5 in CD138+ cells from MM patients (n=25) and normal volunteers (NCs, n=25) were detected by qRT-PCR. (C) The expression level of ALKBH5 in MM cell lines (RPMI8226, ARH-77, NCI-H929 and U266 cells) were examined by western blot. (D) The expression level of ALKBH5 in different stages of MM patients was compared by qRT-PCR. The images shown are representative of three independent experiments. (E) The expression level of ALKBH5 in MM cell lines (RPMI8226, ARH-77, NCI-H929 and U266 cells) were examined through qRT-PCR. *P<0.5, **P<0.01, ***P<0.001.