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. 2022 Apr 8;20(4):e07204. doi: 10.2903/j.efsa.2022.7204

Table 6.

Specifications of the NF

Description: Beta‐lactoglobulin is a white to cream powder produced from bovine whey by crystallisation under acidic or neutral conditions.
Source: Bovine whey
Parameter Specification
Acidic conditions Neutral conditions Combined
Physico‐chemical parameters
Insolubility index (mL) ≤ 0.3 ≤ 0.3 ≤ 0.3
pH (10% solution) 3.5–4.0 6.0–8.0 3.5–8.0
Composition
Protein ‘as is’ (N × 6.38) (%) ≥ 86.0 ≥ 86.0 ≥ 86.0
Protein in DM(1) (N × 6.38) (%) ≥ 90.0 ≥ 90.0 ≥ 90.0
BLG (% of protein) ≥ 90.0 ≥ 90.0 ≥ 90.0
Lactose (%) ≤ 0.2 ≤ 1.0 ≤ 1.0
Fat (%) ≤ 0.5 ≤ 1.0 ≤ 1.0
Ash (%) ≤ 4.5 ≤ 5.0 ≤ 5.0
Moisture (%) ≤ 5.5 ≤ 5.5 ≤ 5.5
Minerals
Calcium (%) ≤ 0.05 ≤ 0.15 ≤ 0.15
Chloride (%) ≤ 1.9 ≤ 1.0 ≤ 1.9
Magnesium (%) ≤ 0.1 ≤ 0.1 ≤ 0.1
Phosphorus (%) ≤ 0.03 ≤ 0.03 ≤ 0.03
Potassium (%) ≤ 0.1 ≤ 1.2 ≤ 1.2
Sodium (%) ≤ 0.1 ≤ 0.5 ≤ 0.5
Contaminants
Cadmium (mg/kg) < 0.2 < 0.2 < 0.2
Lead (mg/kg) < 0.1 < 0.1 < 0.1
Mercury (mg/kg) < 0.01 < 0.01 < 0.01
Aflatoxin M1 (µg/kg) < 0.01 < 0.01 < 0.01
Microbial parameters
Total plate count (CFU/g) ≤ 5,000 ≤ 5,000 ≤ 5,000
Yeast and mould (CFU/g) < 10 < 10 < 10
Enterobacteriaceae (CFU/g) < 10 < 10 < 10
Salmonella (in 25 g) ND ND ND
B. cereus (CFU/g) < 100 < 100 < 100
L. monocytogenes (in 25 g) ND ND ND
S. aureus (CFU/g) < 10 < 10 < 10
Sulfite‐reducing clostridia (CFU/g) < 10 < 10 < 10

BLG: Beta‐lactoglobulin; CFU: Colony forming unit; DM: Dry matter; ND: Not detected.

(1): Protein in DM = (Protein ‘as is’/DM) ? 100%.