TABLE 3.
Spontaneous HSV mutation frequency as scored by plating efficiency using HPMPC
| Virus type | Strain | HPMPC IC50 (μg/ml)
|
Mean mutation frequency to HPMPC (8 μg/ml)a | Mutation frequency range (10−3)c | % Resistant virus | |
|---|---|---|---|---|---|---|
| Mean ± SD | Range | |||||
| HSV-1 | SC16 | 0.65 ± 0.12 | 0.50–0.90 | 9.1 × 10−5 | 0.07–0.12 | 0.009 |
| HSV-2 | SB5 | 0.98 ± 0.13 | 0.90–1.3 | 2.2 × 10−3 | 1.1–4.4 | 0.20 |
| 333 | 0.80 ± 0.14 | 0.60–1.0 | 5.6 × 10−3 | 3.6–7.3 | 0.60 | |
| 6757 | 0.72 ± 0.20 | 0.30–0.90 | 2.8 × 10−2 | 21.0–36.0 | 2.8 | |
| 6652 | 1.00 ± 0.35 | 0.70–1.8 | 5.1 × 10−3 | 2.4–7.0 | 0.50 | |
| 64C | 0.83 ± 0.17 | 0.60–1.1 | 7.7 × 10−3 | 4.9–11.4 | 0.80 | |
| 83D | 0.75 ± 0.13 | 0.50–0.90 | 2.9 × 10−3 | 1.8–4.0 | 0.30 | |
| Meanb | 4.7 × 10−3 | 0.48 | ||||
a
These values are means of 10 replicate wells from two independent experiments.
b
The HSV-2 mean was calculated without isolate 6757, clearly an atypical isolate with an unusually high mutation frequency.
c
Range represents the low and high frequency of resistance calculated from each test sample, the number of drug-resistant viruses per total number of viruses.