FIGURE 2.

Neutrophils in breast cancer (BC) have an immunosuppressive phenotype, which is associated with serum amyloid A 1 (SAA1) expression. (A) Correlation between SAA1 expression level and infiltration level of neutrophils was analyzed by the TIMER 2.0 database. (B) Positive rates of infiltration of neutrophils in BC by TIMER 2.0 database. (C) Flow cytometry analysis of neutrophils (CD11b⁺CD15⁺) isolated from BC patients. (D) Plasma expression levels of interleukin‐10 (IL‐10), arginase (Arg), and inducible nitric oxide synthase (iNOS) in neutrophils from BC patients and healthy controls. (E) Representative images (left) for positive and negative expression levels of SAA1 and CD66b in BC tissues. Black boxes indicate areas shown at a higher magnification (200×). Quantification data of CD66b⁺ cells in positive and negative expression levels of SAA1, expressed as number of immunohistochemistry positive cells per field. Scale bar = 50 μm. (F) Correlation analysis between SAA1 expression in plasma and IL‐10, Arg, and iNOS expression levels in neutrophils from BC patients. Data are expressed as mean ± SD. **p < 0.01