Figure 5.

PLX5622 inhibits BM monocyte production. (A, B) Number of infiltrating brain cells (A) and microglia (B) at dpi 7 in mouse groups fed low-dose PLX5622: Ctrl, PLX^lo-Ctrl and PLX^lo . (C) Correlation between the frequency of BrdU⁺ mature BM monocytes out of CD45⁺ BM cells and the number of CNS infiltrating Ly6C^hi macrophages at dpi 7. (D) Correlation between the number of mature BM monocytes and the number of CNS infiltrating Ly6C^hi macrophages at dpi 7. (E–G) Number of myeloid cells in the BM of mock- and WNV-infected mice (E), mock-infected PLX and Ctrl mice (F) and infected PLX, PLX-Ctrl and Ctrl mice (G). (H) tSNE plot clustered on BM cells from PLX, PLX-Ctrl or Ctrl mice at dpi 7. (I) tSNE plots showing the expression of BrdU and CD115 in/on BM cells from PLX, PLX-Ctrl or Ctrl mice at dpi 7. (J–L) Number and frequency of BrdU⁺ mature BM monocytes in mock and infected mice (J), mock-infected PLX and Ctrl mice (K) and infected PLX, PLX-Ctrl and Ctrl mice (L). Data is presented as mean ± SEM from one (A, B, F, K) or three (E, G, H–J, L) independent experiment with at least three mice per group.