Figure 7.

Anti-CSF-1R does not affect monocyte trafficking into the CNS. (A) Schematic of experimental workflow. Mice were infected and treated with an isotype control monoclonal blocking antibody or anti-CSF-1R. Two hours prior to tissue collection animals were injected with an intravenous dye. (B) Number of myeloid and lymphoid populations in brains of mice infected with WNV and treated with an isotype control antibody or anti-CSF-1R. (C) Number of PKH26⁺ Ly6C^hi macrophages infiltrating the WNV-infected brain per minute at dpi 7. (D) Percent of PKH26⁺ Ly6C^hi macrophages in WNV-infected brains at dpi 7. (E, F) Number (E) and percent (F) of myeloid cells in infected (dpi 7) BMs from mice treated with an isotype control or anti-CSF-1R. (G, H) Number (G) and percent (H) of BrdU⁺ myeloid cells in infected (dpi 7) BMs from mice treated with an isotype control or anti-CSF-1R. Data is presented as mean ± SEM from one independent experiment with at least three mice per group.