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. 2022 Mar 25;16:796129. doi: 10.3389/fnins.2022.796129

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Copyright © 2022 Serrano, Kim, Petrinovic, Turkheimer and Cash.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative hippocampal labeling of the synaptic vesicle protein synaptotagmin-1 in a control (A) and (B) epilepsy [kainic acid rat model of temporal lobe epilepsy (Levesque and Avoli, 2013)]. Both images were obtained using the polyclonal rabbit anti-Syt1 (Abcam, Cambridge, MA; Cat#ab131551); dilution 1:100 (overnight, 4°C). The secondary antibody was donkey anti-Rabbit Alexa Fluor488-conjugated (Thermo Fisher Scientific, Oregon, United States; Cat#A-21206); dilution 1:500 (45 min, RT). DAPI was used to counterstain (blue). The images were obtained with a scanning laser microscope (Leica TCS SP5 with AOBS, Leica Microsystems IR GmbH, Germany) with a 20 × magnification and similar exposure time. Note a decrease in synaptotagmin-1 labeling in the epileptic rat (3 months after kainic acid administration), compared to the control. Images were obtained at GIGA-CRC in vivo imaging and the GIGA-Imaging platform, ULiège (Belgium).