Table 2.
Antibodies used to characterize the neutrophil subpopulations.
| Antibody | Clones | Label | Vol per test (~106 cells) or concentration | Reference | Manufacturer |
|---|---|---|---|---|---|
| Bluevid | BUV736 | 1X | L23105 | Lifetechnologies | |
| CD64 | 10.1 | BUV737 | 3 μL | 612776 | BD |
| CD11b | REA713 | FITC | 2 μL | 130-110-552 | Milteny |
| CD45 | REA599 | Viogreen | 2 μL | 130-177-193 | Milteny |
| CD3 | SP34.2 | BV650 | 2 μL | 563916 | BD |
| CD8 | RPAT8 | BV650 | 2 μL | 563821 | BD |
| CD20 | 2H7 | BV650 | 2 μL | 563780 | BD |
| CD123 | 7G3 | BV650 | 5 μL | 563405 | BD |
| CD62L | SK11 | BV711 | 2 μL | 565040 | BD |
| CD14 | REA599 | Vioblue | 2 μL | 130-110-524 | Milteny |
| CD10 | HI10a | PercP-Cy5,5 | 5 μL | 312216 | Biolegend |
| CDw125 | REA705 | PE | 2.5 μL | 130-110-544 | Milteny |
| PD-L1 | 29E.2A3 | PE-Dazzle594 | 3 μL | 329732 | Biolegend |
| CD101 | REA954 | PE-Vio770 | 2 μL | 130-115-832 | Milteny |
| CD32a | IV.3 | AF647 | 0.5 mg/mL | 60012 | Stemcell |
| HLA-DR | L234 | AF700 | 1 μL | 307626 | Biolegend |
| CD66 | TET2 | APC-Vio770 | 2 μL | 130-119-847 | Milteny |