TABLE 2.

Primers used to study M. leprae folP1 and folP2

Primer	Sequencea	Location (bp)	Application(s)
folP1-1	5′ TGATGCTGCTTCTCGTGC 3′	28–45 upstream of GTG start	PCR, sequencing of folP1
folP1-2	5′ AAGTCTTGTCCGTTGGCG 3′	61–78 downstream of TAG stop
folP1-9	5′ AGGCCGTGCTGGACAGC 3′	91–108	Sequencing of folP1
folP1-20	5′ CGTCAACGATGTGTCT 3′	308–323
folP1-7	5′ CCGGAATTCCGTGAGTTTGGCG 3′	1–12 + EcoRI site	PCR, RT-PCR, cloning of folP1
folP1-8	5′ GCGGGATCCGTCAGCCATCACATC 3′	842–856 + BamHI site
folP1-21	5′ CCGGTGCCATTAGGACCGA 3′	164–182	Site-directed mutagenesis of folP1 (T53I)
folP1-22	5′ GCCGGATCGATTCGCCACCG 3′	147–163
folP1-23	5′ CGACCCCGGCGCGGTGCCAT 3′	155–173	Site-directed mutagenesis of folP1 (P55R)
folP1-24	5′ ATTCGCCACCGACGTCGA 3′	137–154
folP2-1	5′ CCGGAATTCCGTGCAGTCAAT 3′	1–11 + EcoRI site	PCR, RT-PCR, cloning of folP2
folP2-2	5′ GCGGGATCCGTCATGCGAGT 3′	866–878 + BamHI site
folP2-3	5′ GATGTGATTCGCCAGG 3′	492–507	Sequencing of folP2
folP2-4	5′ CGAATCACATCGTCCACCAC 3′	483–502
EC2	5′ TGGAATTCTTATCAATTCATACCAGGG 3′	35–9 upstream of ATG start	PCR of E. coli folP
EC3	5′ GGAGATCTACACGACCACGAATCCCATC 3′	20–47 downstream of TAA stop

^aMutated nucleotides are underlined.