TABLE 3.
Growth, DHPS activity, and dapsone inhibition of E. coli C600 and recombinant strains
| E. coli strain | Growtha | DHPS activity (pmol/mg)b | Dapsone IC50c (μg/ml) | Dapsone MICd (μg/ml) |
|---|---|---|---|---|
| C600 | + | 370 ± 16.6 | 3.0 | >256 |
| C600ΔfolP::Kmr | − | 9.8 ± 1.2 | ND | ND |
| C600ΔfolP::Kmr(pUC18) | − | 10.3 ± 0.8 | ND | ND |
| C600ΔfolP::Kmr(pML101) | + | 48.7 ± 4.2 | 0.06 | 1 |
| C600ΔfolP::Kmr(pML201) | − | 5.5 ± 0.3 | ND | ND |
| C600ΔfolP::Kmr(pML102) | + | 62.1 ± 1.4 | 0.11 | 10 |
| C600ΔfolP::Kmr(pML103) | + | 60.9 ± 5.4 | 4.1 | 64 |
a
Bacterial growth on Mueller-Hinton agar. +, growth; −, no growth.
b
Values are means ± standard deviations.
c
Dapsone concentration inhibiting 50% of DHPS activity. ND, not determined.
d
On Mueller-Hinton agar.