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. 2022 Mar;11(3):475–487. doi: 10.21037/tcr-21-2903

Figure 2.

Figure 2

LncRNA01977 knockdown decreased the proliferation, metastasis, and chemochemical resistance to DOX in LUAD cells. (A) The efficiency of shRNA knockdown was assessed by real-time PCR in HCC-78, KTA-7 and PC-9 cells. (B) The MTT assay was used to assess the cell viability of HCC-78, KTA-7 and PC-9 transfected with a control or knockdown plasmid. (C) The colony formation assay showed the effect of LncRNA01977 knockdown on LUAD cell proliferation. (D) DOX IC50 in the control plasmid or HCC-78, KTA-7 and PC-9 transfected with the knockdown plasmid. (E) The cytotoxicity assay was used to test the inhibition of DOX resistance caused by LncRNA01977 knockdown in HCC-78, KTA-7 and PC-9 cells. DOX concentration: 0.1 M in HCC-78, KTA-7 and PC-9 cells, 0.5 M in MCF-7 cells. (F) Cell migration and invasion assay [Cell HE staining]. (G) Cell scratch assay (*, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001). LUAD, lung adenocarcinoma; DOX, Docetaxel; TCGA, The Cancer Genome Atlas; PCR, polymerase chain reaction; MTT, Methyl tetrazolium; HE, hematoxylin-Eosin; NC, normal contras; ns, meaningless. (Observational method: High power microscope ×45).