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. 2022 Mar 25;12:765553. doi: 10.3389/fphar.2021.765553

FIGURE 5.

FIGURE 5

Inhibition of cytokine release level by the TCM candidates. After 6 and 24 h of honeysuckle-EtOH (A) or APS-L (B) treatment in the presence or absence of LPS, THP-1 cell medium was collected, and ELISA was performed to measure cytokine levels. Treatment with LPS alone was considered to be the control. (C) After 24 h of honeysuckle-EtOH treatment in the presence or absence of stimulators (LPS, Spike, LPS + Spike, and R848), THP-1 cell medium was collected, and ELISA was performed to measure IL-6 levels. Data are expressed as mean ± SD from three repeated results and analyzed using Student’s t-test. * compared to LPS stimulation alone, # compared to combined stimulation of LPS and Spike, and $ compared to R848 stimulation alone. *, significantly different from the corresponding control, at p < 0.05; **, p < 0.01. Similar for # and $.