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. 2022 Feb 14;113(4):1338–1351. doi: 10.1111/cas.15293

FIGURE 1.

FIGURE 1

The effects of CXCL12 on CCR7 ligand–dependent MDA231 cell migration. A, CXCL12‐induced cell migration assay was performed using MDA231 cells in the presence or absence of CXCL12 at indicated concentrations. The number of migrated cells to the lower wells in response to CXCL12 was analyzed. Graphs represent means ± SD of the number of cells in eight random fields per membrane in triplicate assay. * p < 0.05 by Student's t test. B, CCL21‐induced cell migration of MDA‐MB‐231. The assay was performed in the presence of CCL21 in the lower wells at indicated concentrations. One‐way ANOVA; *p < 0.05, **p < 0.01. C, The effect of CXCL12 on MDA231 cell migration induced by a suboptimal concentration of CCL21 (50 ng/mL). The assay was performed under the indicated concentrations of CXCL12 and CCL21 added to the upper or the lower wells. One‐way ANOVA; **p < 0.01. D, The effect of CXCL12 on CCL21‐induced cell migration in the presence of anti‐CXCR4 neutralizing antibody. CXCL12 (2.5 μg/mL) was added to the upper wells with or without anti‐CXCR4 mAb or an isotype control immunoglobulin (10 μg/mL). Mean ± SD (Student's t test; *p < 0.05, **p < 0.01, n = 3)