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Localization of CCR7 and CXCR4 in the lipid rafts of ruffling membrane. A, CCR7 (green) and CXCR4 (red) were detected in cells expressing CCR7‐EGFP and CXCR4‐mCherry. GM1 (blue) was detected with Alexa Fluor647‐labeled CTxB. B, CD44 (blue) was detected with a biotinylated anti‐human CD44 mAb followed by Alexa Fluor647–conjugated streptavidin. White arrowheads represent ruffling membrane
