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. 2022 Mar 25;9:742193. doi: 10.3389/fcvm.2022.742193

Figure 1.

Figure 1

Experimental design of the two-hit models of latent DOX cardiotoxicity using ANGII (A) or ISO (E) as second hits. Male 5-week old mice were administered DOX (4 mg/kg/week) or saline for 3 weeks and allowed to recover for 5 weeks prior to exposure to ANGII infusion (1.4 mg/kg/day for 14 days) or ISO injections (10 mg/kg/day for 14 days). Hypertrophic response to ANGII and ISO is abrogated by juvenile exposure to DOX. (B,F) Heart weight to tibial length ratio (HW/TL) (n = 6-9 per group). (C,G) Representative heart sections. (D,H) Quantification of cardiomyocyte surface area; bar scale = 50μM. Values are represented as means ± SEM. Statistical significance of pairwise comparisons was determined by two-way ANOVA with Tukey's post-hoc analysis (*p < 0.05, **p < 0.01). ANGII, Angiotensin II; DOX, doxorubicin; ISO, isoproterenol.