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. 2022 Mar 24;12:865686. doi: 10.3389/fonc.2022.865686

Figure 2.

Figure 2

The Kv1.3 interactome reveals an unconventional mitochondrial import pathway for Kv1.3. (A) Network showing protein–protein interactions (PPIs) in the mitochondrial Kv1.3 interactome of HEK 293 cells transfected with Kv1.3YFP. The mitochondrial import subnetwork was obtained after a functional enrichment analysis of the Kv1.3 interactome. A force-directed layout was used for network visualization. Protein names are indicated at the node center. The color code indicates functional relations between proteins (purple, chaperones; blue, matrix proteases; red, PAM complex; orange, TIM23 complex; green, TOM complex; yellow, MICOS complex). TOMM34, CDC37 and YWHAE were manually annotated based on published evidence. (B) HEK 293 cells were transfected with Kv1.3YFP. Total cell lysates (SM, starting materials) were immunoprecipitated against Kv1.3 (IP+). IP-, absence of the Kv1.3 antibody. Samples were immunoblotted (IB) with antibodies against Kv1.3 and different TIM/TOM proteins, as indicated.