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. 2022 Mar 24;12:865686. doi: 10.3389/fonc.2022.865686

Figure 4.

Figure 4

Transmembrane domains are candidate determinants of the mitochondrial import of Shaker channels. HEK 293 cells were transfected with Kv1.1-Kv1.5YFP channels, and mitochondrial expression was analyzed. (A) Membranous (Mb) and mitochondrial (Mit) fractions of HEK 293 cells transfected with the indicated member of the Shaker family. Note a shift in the molecular weights of the Kv1.2, Kv1.3 and Kv1.5 channels between the Mb and Mit fractions. Na+/K+ ATPse is used as a plasma membrane marker, and VDAC is used as a mitochondrial marker. (B) Multiple protein sequence alignment of members of the Shaker family of voltage-gated potassium channels performed using Clustal Omega and JalView software. UniprotKB reference numbers are indicated. Hydrophobic residues are indicated in red, and hydrophilic residues are indicated in blue. Conservation among sequences is indicated in yellow. S1-S6 black lines indicate the sequence fragments corresponding to the transmembrane segments.