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. 2022 Jan 1;29(4):861–873. doi: 10.1038/s41418-021-00901-0

Fig. 2. OGA interacts with MORC2 and de-O-GlcNAcylates MORC2 at T556.

Fig. 2

A, B HEK293T cells were transfected with HA-MORC2 and Flag-OGA alone or in combination. IP and immunoblotting analysis were performed with the indicated antibodies after 48 h of transfection. C, D Lysates from MCF-7 and T47D cells were subjected to IP and immunoblotting analysis with the indicated antibodies. E HEK293T cells were transfected with Flag-OGA and HA-MORC2 alone or in combination. IP and immunoblotting analysis were performed with the indicated antibodies after 48 h of transfection. O-GlcNAc levels were normalized to levels of immunoprecipitated HA-MORC2. F HEK293T cells transfected with pCMV or Flag-MORC2 were treated with or without 20 μM PUGNAc for 24 h and subjected to IP and immunoblotting analyses with the indicated antibodies. O-GlcNAc levels were normalized to levels of immunoprecipitated Flag-MORC2. G, H MCF-7 and T47D cells were treated with or without 20 μM PUGNAc for 24 h and subjected to IP and immunoblotting analyses with the indicated antibodies. O-GlcNAc levels were normalized to levels of immunoprecipitated MORC2. I, J MCF-7 and T47D cells were transfected with siNC or two different siRNAs targeting OGA (siOGA). After 48 h of transfection, cells were subjected to IP and immunoblotting analysis with the indicated antibodies. In I, O-GlcNAc levels were normalized to levels of immunoprecipitated MORC2. K HEK293T cells were transfected with the indicated expression vectors. After 24 h of transfection, cells were treated with or without 20 μM PUGNAc for 24 h and subjected to IP and immunoblotting analysis. O-GlcNAc levels were normalized to levels of immunoprecipitated Flag-MORC2.