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Microbiological assay plate showing inactivation of imipenem (central disk) by the A. baumannii OXA-24 enzyme. (1) Twenty microliters of semipurified OXA-24 protein (nitrocefin-specific activity, 251.5 μmol/min/μl); (2) 10 μl of the same extract; (3) 5 μl of the same extract; (4) 20 μl of phosphate-buffered saline.
