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. 2022 Apr 7;13:1897. doi: 10.1038/s41467-022-29499-8

Fig. 2. FGF21 mediates low protein-induced changes in BW gain, food intake, and body composition in aged mice.

Fig. 2

a Schematic representation of the metabolic aging study. Wildtype and Fgf21-KO mice were placed on control or LP diet at 3 months of age (12 mice/group), with various metabolic endpoints assess throughout the study as indicated, and tissue collection at 22 months of age. b Body weight gain over time following initiation of experimental diets, n = 12 mice/group, group*time p = 0.001. c Final body weight gain at 22 months of age (n = 8–12 mice/group at sacrifice; geno*diet p = 0.0004). d Final body fat gain at 22 months of age as measured by NMR (n = 8–12 mice/group at sacrifice; geno*diet p = 0.03). e Final body lean gain at 22 months of age as measured by NMR (n = 8–12 mice/group at sacrifice; geno*diet p = 0.0001). f Percent body fat at 22 months of age as measured by NMR (n = 8–12 mice/group at sacrifice; geno*diet p = 0.03). g Average daily food intake throughout the study (n = 8–12 mice/group at sacrifice; geno*diet p = 0.0001). h Energy expenditure (ANCOVA adjusted for BW) as measured at 12 months of age (n = 8–12 mice/group at sacrifice; geno*diet p = 0.04). i Energy expenditure (ANCOVA adjusted for BW) as measured at 20 months of age (n = 8–12 mice/group at sacrifice; geno*diet p = 0.04). Statistical analyses were conducted using two-way ANOVA or ANCOVA. All values are mean ± SEM, with significant posthoc comparison within the diet*genotype interaction noted as *p < 0.05 compared with respective control. Source data are provided as a Source Data file.