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. 2022 Apr 7;13:1897. doi: 10.1038/s41467-022-29499-8

Fig. 5. FGF21-dependent and independent changes in molecular endpoints in the liver at 22 months of age.

Fig. 5

a Serum FGF21 levels collected at sacrifice at 22 months of age in WT and Fgf21 KO mice on control or LP diets as in Fig. 2 (n = 8–10 mice/group, geno*diet p = 0.01). b Liver mRNA levels for FGF21 related markers as measured via qPCR at 22 months of age (n = 8–10 mice/group, geno*diet Fgf21 p = 0.04, Fgfr1 p = 0.556, Klb = 0.3016). c Liver markers of dietary protein restriction (amino acid biosynthetic genes) as measured via qPCR at 22 months of age (n = 8–10 mice/group; geno*diet Asns p = 0.0001, Phgdh p = 0.001. d Serum IGF1 levels at sacrifice at 22 months of age (n = 8–10 mice/group; genotype p = 0.0004, diet p = 0.003, geno*diet p = 0.4567). e Liver mRNA expression for hepatic lipogenic genes as measured via qPCR at 22 months of age (n = 8–10 mice/group; Scd1 diet p = 0.001 geno*diet p = 0.553, Srebp1 diet p = 0.001, geno*diet p = 0.89, Fas diet p = 0.001 geno*diet p = 0.2395. f Liver weight as a percentage of body weight at sacrifice at 22 months of age (n = 8–10 mice/group; diet p = 0.0026, geno*diet p = 0.10). g Liver triglyceride content at sacrifice at 22 months of age (n = 8–10 mice/group, geno*diet p = 0.2271). h Serum ALT levels at sacrifice at 22 months of age (n = 8–10 mice/group; genotype p = 0.01, diet p = 0.0003, geno*diet p = 0.0057). i Serum AST levels at sacrifice at 22 months of age (n = 8–10 mice/group; genotype p = 0.6662, diet p = 0.02, geno*diet p = 0.2852). Statistical analyses were conducted using two-way ANOVA. All values are mean ± SEM, with significant main effects of diet or posthoc comparison within the diet*genotype interaction noted as *p < 0.05, #p < 0.10 compared with respective control. Source data are provided as a Source Data file.