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. 2022 Apr 7;13:1906. doi: 10.1038/s41467-022-29549-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic for quantifiable visual detection based on the distance signal readout. b Time-dependent marker advancement with different concentrations of Phe-Gly-Gly. c Linear standard curves obtained from 0 to 100 μM Phe-Gly-Gly in 1 min. Inserts: Images showing marker advancement at various concentrations of Phe-Gly-Gly in 1 min. d Quantifiable visual CO₂ release based on the color signal readout. Left: Plots of CO₂ emission against the Phe-Gly-Gly concentration. Right: Illustration of the color signal readout by the HG-LGS. Error bars represent standard deviations (n = 3).