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. 2022 Apr 7;8:174. doi: 10.1038/s41420-022-00976-9

Fig. 2. Blocking MOXD1 inhibits GBM cell proliferation and induces cell arrest.

Fig. 2

A After MOXD1 knockdown by shRNA in GBM cell lines (LN-229, U87 MG), MOXD1 expression was detected using qRT-PCR. B After MOXD1 knockdown by shRNA in GBM cell lines (LN-229, U87 MG), MOXD1 expression was detected using Western blot analysis. C One thousand cells were tiled in a six-well plate and the morphology of the cells was observed after culturing for 48 h. D One thousand cells are flattened into a six-well plate, and the number of cells is counted after culturing for 48 h. E MOXD1 knockdown inhibited the proliferation of LN-229 and U87 MG cells, MTT assay was used to examine the effect of MOXD1 knockdown on cell viability. F BrdU assays was used to detect the DNA synthesis ability after MOXD1 knockdown. G The statistics of the amount of DNA synthesis of LN-229 and U87 MG cells ability after MOXD1 knockdown. H Cell cycle was assessed by flow cytometry after MOXD1 knockdown. I Statistics of each cycle of the cell after MOXD1 knockdown. J Western blot assay was executed to detect the expression of G2/M cell cycle regulatory proteins in MOXD1 knockdown cells.