Table 3.

qPCR cycling conditions

Steps	Temperature	Time	Cycles
Start-up	4°C	2 min
Initial Denaturation	95°C	15 min	1
Denaturation	95°C	15 s	40 cycles
Annealing	60°C	1 min
Extension	95°C	30 s
Melt Curve	65°C–95°C	0.5 °C/5 s
Hold	25°C	5 min